Life Sciences 3 Introduction to Molecular Biology
Information for Laboratory Instructions 
   
Contacts for lab instructions  
  Lab Director /Development  
  Gaston Pfluegl gaston@lifesci.ucla.edu Slichter 2875 794-4113  
  Administrative issues  
  Tracy Newman tracyn@lifesci.ucla.edu LS 2305  825-6614  
  Lily Yanez lyanez@lifesci.ucla.edu LS 2305  825-6614  
  Jane Park jpark@lifesci.ucla.edu LS 2305 825-6614  
   
Lab topics  
  Labs are held on Tuesday through Friday of Weeks 2, 4, 6, 8, and 10.  Assignments are due at the end of lab.   
   
  Week Activity Assignment Points  
  (quiz +) lab  
  2 Lab 1 - Pipetting/SDS-PAGE and Proteins In lab 20  
  4 Lab 2 - β-galactosidase Assay In lab (5 +) 15  
  6 Lab 3 - DNA Isolation and Amplification In lab (5 +) 15  
  8 Lab 4 - Agarose Gel Electrophoresis In lab (5 +) 15  
  10 Lab 5 - Sequence Analysis In lab (5 +) 15  
  Drop lowest lab score - NOT Lab 3 - 20  
  Lab questions for final 20  
  Total  100  
   
Lab manuals  
  The 10th Edition of the lab manual is required for 2009-10.  It is available for TAs at the LS Core office.  Students can purchase them at the campus bookstore.    
   
Lab grade transfer  
  Students who took the course within the last four sessions (including summer) are allowed to transfer their previous grades from the lab portion and be exempt from attending the labs.  All students in the course must take the lab portion of the final exam.  The policy and sign up procedure are available after students log on to http://www.lsic.ucla.edu/classes/  and click on the link under the “Announcements”.  Please remind students of this option at the first discussion or lab.  
   
Make-up Labs, Switching Sections, Lab Re-grades  
  Make-up labs will not be granted.  If a student has a legitimate, excused absence (with documentation), lab points can be pro-rated.  Information on obtaining documentation for an absence due to illness can be found on the Ashe Center website at www.studenthealth.ucla.edu.  Once enrolled, students are not allowed to switch sections to accommodate their schedules.  Requests for lab assignment re-grades must be submitted within one week of the return of the assignment, in writing, and will be considered at the end of the term.  
   
Quizzes   
  Quizzes are given in labs 2, 3, 4 and 5.  Each is worth 5 points.   
   
LS Laboratory Website  
  Information on class, section and room schedule can be found here:  
  http://lslab.lscore.ucla.edu